ASCP-MLT

Caffeine benzoate solution is used to split the unconjugated bilirubin protein complex releasing the bilirubin so that it can react with diazotised sulphanilic acid. The tartrate buffer creates an alkaline solution and converts the red acid bilirubin to a green coloured compound which can be measured spectrophotometrically.

Which substance is used in the Jendrassik-Grof method to accelerate the reaction of unconjugated bilirubin with the diazo reagent?
A . NADH
B. N-butanol
C. caffeine
D. acetic acid

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Answer: B

Set of comprehensive safety guidelines designed to protect patients and health care workers
A . Standard Precautions
B. Universal blood precautions
C. Microscope
D. Transmission precautions

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Answer: A

The laboratory uses flammable chemicals for processing and staining patient specimens.

One type of chemical used is ethanol.

What is the proper way to store this chemical in the laboratory?
A . On the shelf with other hazardous chemicals
B. Under the sink in the laboratory
C. In a flame-resistant cabinet
D. In the back of the fume hood

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Answer: C

What is the best description of the purpose of the Commission on Office Laboratory Accreditation (COLA) pertaining to the clinical laboratory?
A . Administers both CLIA ’88 and Medicare programs
B. Nonprofit educational group that establishes consensus standards for maintaining a high-quality laboratory organization
C. CMS has given COLA deemed status to act on the government’s behalf ot certify clinical laboratories
D. Sets accreditation requirements for physician office laboratories (POLs)

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Answer: A

As magnification DECREASES, the opening of the iris diaphragm will…
A . stay the samel
B. decrease (get smaller)
C. increase (get bigger)

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Answer: B

High triglycerides may be caused by disorders such as type 2 diabetes, hypothyroidism, Cushing’s sydnrome, liver disease, uremia, dysglobulinemia, nephrotic syndrome, and alcoholism can cause hypertriglyceridemia.

A 46-year old known alcoholic with liver damage is brought in the ER unconscious. One would expect his lipid values to be affected in what way?
A . Increased
B. Decreased
C. Normal
D. Unaffected by the alcoholism

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Answer: A

The results of this PT and aPTT are in normal range. These results can be reported and are not indicative of the need to: order a mixing study or request a redraw.

You have just performed stat PT and aPTT tests on your coagulation instrument.

Your results are as follows:

PT = 12 seconds (normal range 10-13 seconds)

aPTT = 24 seconds (normal range 21-34 seconds)

What would be your next step?
A . Perform a mixing study
B. Report the results
C. Request a redraw of the specimen

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Answer: B

Which of the following legislation was developed to protect the privacy of all patient information in the health care?
A . Health Insurance Portability and Accountability Act (HIPPA)
B. Occupational Safety and Health Administration (OSHA)
C. Clinical Laboratory Standards Institute (CLSI)
D. Clinical Laboratory Improvement Amendments (CLIA)

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Answer: A

A laboratory scientist is working the night shift at a local hospital when the power goes out.

What is the course of action to continue to provide laboratory results?
A . Wait until the power comes back
B. Hold all laboratory testing until the power comes back
C. Initiate the downtime protocol for an outage
D. Wait until the physician calls asking for results

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Answer: C

Bacitracin susceptibility is a presumptive test for the identification of Lancefield group A Streptococcus.

Which of the following is a presumptive test for the identification of Lancefield group A Streptococcus:
A . Bacitracin susceptibility
B. Positive coagulase
C. Hippurate hydrolysis
D. Gram stain

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Answer: A